Independent and Epigenetic Regulation of the Interleukin-4 Alleles in CD4 + T Cells

Abstract

How an individual effector T cell acquires a particular cytokine expression pattern from many possible patterns remains unclear. CD4⁺ T cells from F₁ mice, which allowed assignment of the parental origin of interleukin-4 (IL-4) transcripts, were divided into clones that expressed IL-4 biallelically or monoallelically from either allele. The allelic pattern was transmitted as a stable epigenetic trait. Regulation of cytokine expression by a mechanism that treats each allele independently suggests a probabilistic process by which a diverse repertoire of combinatorially assorted cytokine gene expression patterns could be generated among the clonally related daughters of a single precursor cell.