Hemagglutination and Hemagglutination-Inhibition with Coxsackie B Viruses

Abstract

Agglutination of human erythrocytes by Coxsackie B virus types 1 and 5 is reported. Optimum titers were obtained with a sedimentation temperature of 37[degree]C and the erythrocytes of newborn infants. A hemagglutination-inhibition (HI) procedure employing Coxsackie B virus types 1, 3, and 5 was used to test paired sera from 25 children infected with Coxsackie B virus types 3, 4, and 5. A significant homologous response was detected in every case except one. A few hetero-logous responses occurred in children with pre-existing HI antibody to one or more of the 3 test antigens.