Differential mRNA stability to reticulocyte ribonucleases correlates with 3′ non‐coding (U)nA sequences
Open Access
- 3 March 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 172 (2), 333-340
- https://doi.org/10.1111/j.1432-1033.1988.tb13891.x
Abstract
The stabilities of different mRNA species were analyzed in a reticulocyte lysate system under protein-synthesizing conditions. In all cases examined the relative mRNA degradation by reticulocyte ribonucleases as well as by the interferon-modulated (2′-5′) (A)n-dependent endonuclease correlated with the extent of (U)nA sequences within the 3′ non-coding region. The experimental data presented indicate that according to their stabilities at least three major mRNA groups may be identified: (a) (U)nA-poor mRNAs (e.g. globin) are essentially stable and are only slightly degraded by the (2′-5′)(A)n-dependent endonuclease; (b) mRNA species with intermediate (U)nA levels (e.g. Igα and Igμ heavy-chain mRNAs) are partially degraded by general ribonuclease activity and further degraded by the (2′-5′)(A)n-dependent endonuclease and (c) (U)nA-rich mRNA species (such as c-myc and non-skeletal actin mRNAs) are inherently unstable and are extremely sensitive to degradation by general ribonuclease activity. A survey of mRNA nucleotide sequences demonstrated that without exception (U)nA-rich stretches appeared more frequently within the 3′ non-coding region than in the coding or 5′ non-coding regions. A comparison of 3′ non-coding region sequences from 92 different mRNAs revealed that transiently expressed mRNAs, such as the interleukins, nerve growth factor, epidermal growth factor receptor, c-myc, c-fos, c-myb and several other oncogenes as well as interferons α, β and γ were exceptially (U)nA-rich. It is postulated that differential mRNA stability may be partly determined by the primary nucleotide sequence and in particular by (U)nA sequences within the 3′ non-coding region. This may represent a novel post-transcriptional strategy employed by the cell to selectively retain or destroy discrete mRNA species.This publication has 46 references indexed in Scilit:
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