Nuclear-Magnetic-Resonance Studies of 5'-Ribonucleotide and 5'-Deoxyribonucleotide Conformations in Solution Using the Lanthanide Probe Method
- 1 July 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 88 (1), 259-266
- https://doi.org/10.1111/j.1432-1033.1978.tb12446.x
Abstract
The conformations of the metal-bound 5''-ribonucleotides and 5''-deoxyribonucleotides in aqueous solution at different pH values were studied using the lanthanide probe method. the conformational analysis, based on mixing different conformations in fast exchange within the NMR time scale, agrees well with the results from coupling constants, nuclear Overhauser effects and spin-lattice relaxation times, obtained for the metal-fixed systems. The equilibrium between the 2 basic conformational combinations for the 5''-nucleotides, anti-(N .dblarw. S)-gg-g''g'' and syn-(N .dblarw. S)-gt-g''g'' depends on the nature of the furanose ring, the base and also on the state of base protonation and phosphate ionization. The effect of base protonation is particularly strong for the guanine nucleotides.This publication has 29 references indexed in Scilit:
- Conformational Studies of Some 2′:3′‐Cyclic Mononucleotides in Solution by Different Nuclear‐Magnetic‐Resonance MethodsEuropean Journal of Biochemistry, 1978
- Nuclear magnetic resonance investigation of the conformation of nicotinamide mononucleotide in aqueous solutionJournal of the American Chemical Society, 1975
- Pulsed NMR methods for the observation and assignment of exchangeable hydrogens: Application to bacitracinFEBS Letters, 1974
- Nuclear magnetic resonance studies of 5'-ribo- and deoxyribonucleotide structures in solutionBiochemistry, 1974
- Quantitative determination of conformations of flexible molecules in solution using lanthanide ions as nuclear magnetic resonance probes: Application to adenosine-5′-monophosphateJournal of Molecular Biology, 1974
- Lanthanoid(III) cations as nuclear magnetic resonance conformational probes: studies on cytidine 5′-monophosphate at pH 2J. Chem. Soc., Dalton Trans., 1974
- Ethylenediaminetetra-acetato-lanthanate(III), -praesodimate(III), -europate(III), and -gadolinate(III) complexes as nuclear magnetic resonance probes of the molecular conformations of adenosine 5′- monophosphate and cytidine 5′-monophosphate in solutionJ. Chem. Soc., Dalton Trans., 1974
- Conformational analysis of the sugar ring in nucleosides and nucleotides. Improved method for the interpretation of proton magnetic resonance coupling constantsJournal of the American Chemical Society, 1973
- Effect of pH upon the nuclear magnetic resonance spectra of nucleosides and nucleotidesBiochemistry, 1968
- Studies of inter- and intramolecular interaction in mononucleotides by proton magnetic resonanceJournal of the American Chemical Society, 1968