Neurosecretory-like material in 3rd- and 4th-stageDirofilaria immitislarvae (Nematoda: Filarioidea)
- 1 January 1989
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 99 (1), 99-104
- https://doi.org/10.1017/s0031182000061072
Abstract
SUMMARY: Phase-interference microscopic examination of the infective, post-infective 3rd-stage and 4th-stage larvae ofDirofilaria immitishas identified a single cell body in each of the paired lateral amphidial nerves which undergoes characteristic morphological change during the development from 3rd- to 4th-stage larvae. Acetaldehyde—fuchsin staining of worm sections revealed fuchsinophilic material in the precise location of the amphidial nerve-cell bodies observed by phaseinterference microscopy. This material was found in 70% of infective larvae recovered from mosquitoes and in 100% of larvae recovered from micropore chambers 24 h after implantation into BALB/C mice. In pre-moult larvae recovered at 42 h (Experiment 1) and at 48 h (Experiment 2) fuchsinophilic material was demonstrable, but no staining was observed in those larvae in which separation of the 3rd- and 4th-stage cuticles had occurred. No such material was observed in 4thstage larvae recovered from chambers after 74 h, and in these larvae the amphidial nerve-cell bodies were not discernible. These cytological observations are consistent with a cycle of elaboration and release of neurosecretion associated with moulting inD. immitis.Keywords
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