Isolation of Aleutian Mink Disease Virus by Affinity Chromatography
- 12 January 1973
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 179 (4069), 187-189
- https://doi.org/10.1126/science.179.4069.187
Abstract
Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.Keywords
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