Isolation of Serum Fractions Capable of Producing Aleutian Disease in Mink
- 1 September 1966
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 97 (3), 344-349
- https://doi.org/10.4049/jimmunol.97.3.344
Abstract
Serum protein fractionation of pooled mink serum containing the disease principle was used in an attempt to achieve some relative purification of the agent capable of producing Aleutian disease (AD) in mink. All fractions of zone electrophoretic separations, γ, β, α and albumin as well as the three peaks of Sephadex G-200 separations, produced the disease when injected into normal mink. Some relative purification of serum protein fractions capable of producing AD was achieved using gradient elution diethylaminoethyl (DEAE) column chromatography. Initial fractions did not include the infectious principle, whereas later fractions eluted with pH 5.5–5.0, 0.25–0.3 M phosphate buffers produced the disease in injected mink. Rabbit antisera to DEAE fractions, known to contain infectivity for mink, showed no precipitating antibodies specific for AD serum when absorbed with normal mink serum.This publication has 11 references indexed in Scilit:
- The Experimental Transmission of a Virus Causing Hypergammaglobulinemia in Mink: Sources and Modes of InfectionThe Journal of Infectious Diseases, 1964
- EXPERIMENTAL TRANSMISSION OF ALEUTIAN DISEASE WITH URINE1963
- ALEUTIAN DISEASE OF MINK .1. EVIDENCE OF ITS VIRAL ETIOLOGY1962
- The purification and physicochemical properties of two viruses associated with respiratory diseaseVirology, 1961
- Hypergammaglobulinemia in Mink.Experimental Biology and Medicine, 1961
- Plasma-Cell Hepatitis, with Special Attention to Steroid TherapyArchives of Pediatrics & Adolescent Medicine, 1960
- CHROMATOGRAPHY OF SERUM PROTEINS IN NORMAL AND PATHOLOGIC SERA: THE DISTRIBUTION OF PROTEIN-BOUND CARBOHYDRATE AND CHOLESTEROL, SIDEROPHILIN, THYROXIN-BINDING PROTEIN, B12-BINDING PROTEIN, ALKALINE AND ACID PHOSPHATASES, RADIOIODINATED ALBUMIN AND MYELOMA PROTEINSJCI Insight, 1958
- The purification and chromatography of bacteriophages on anion-exchange celluloseVirology, 1957
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956
- Chromatography of Proteins. II. Fractionation of Serum Protein on Anion-exchange CelluloseJournal of the American Chemical Society, 1956