Efficacy of glycoprotein inhibitors alone and in combination with trifluridine in the treatment of murine herpetic keratitis
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 5 (2), 93-99
- https://doi.org/10.3109/02713688609015097
Abstract
The present study examined the anti-herpetic effect of the glycoprotein inhibitors, hydroxynorvaline and 2-deoxyglucose, alone and in combination with trifluridine on murine ocular herpes. Following ocular inoculation with a large dose of HSV-1 RE strain (106 pfu), ICR mice were treated during the acute infection with different therapeutic regimens, and their efficacy was evaluated by ocular virus titers, clinical grading of blepharo-conjunctivitis and histological evaluation of stromal keratitis and iridocyclitis. The results following a large dose HSV-1 inoculum demonstrated that trifluridine was the best single therapeutic agent. Hydroxynorvaline and 2-deoxyglucose had no effect at all. Combination therapy of the glycoprotein inhibitors with trifluridine was no better than trifluridine alone. The mouse HSV-1 keratitis model proved to be an effective, economical alternative to the rabbit model for the evaluation of new antiviral agents.This publication has 16 references indexed in Scilit:
- Statistical analysis for experimental models of ocular disease: Continuous response measuresCurrent Eye Research, 1985
- Inhibition of synthesis of herpesvirus (HSV-1) glycoproteins and endogenous fusion by β-hydroxynorvaline in BHK-21 cellsVirology, 1984
- In vitro effect of (E)-5-(2-bromovinyl)-2′-deoxyuridine, 5′-amino-5-iodo-2′,5′-dideoxyuridine and 2-deoxy-d-glucose on latent ganglionic herpes simplex virus infectionAntiviral Research, 1984
- Adsorption and Penetration of Enveloped Herpes Simplex Virus Particles Modified by Tunicamycin or 2-Deoxy-D-glucoseJournal of General Virology, 1982
- A study of the antiviral mechanism of action of 2-deoxy-d-glucose: Normally glycosylated proteins are not strictly required for herpes simplex virus attachment but increase viral penetration and infectivityVirology, 1982
- Enzymatic deoxyglucosylation of ceramides by microsomes of BHK-21 cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Herpes simplex virus protein synthesis in the presence of 2-deoxy-d-glucoseVirology, 1976
- A NEW APPROACH TO VIRAL CHEMOTHERAPY: INHIBITORS OF GLYCOPROTEIN SYNTHESISThe Lancet, 1974
- Effects of 2-deoxy-d-glucose on herpes simplex virus replicationVirology, 1973
- The effect of cytosine arabinoside on the replication of herpes simplex virusVirology, 1967