Use of an Antiserum Against Cerebrospinal Fluid in Demonstration of Trace Proteins in Biological Fluids.

Abstract

Rabbit antisera to human cerebrospinal fluid, after "absorption" with human serum, reacted with various human biological fluids. The reactions were studied by immunoelectrophoresis and double diffusion in agar, and revealed the presence of 2 trace proteins of [beta] and gamma electro-phoretic mobility. Both proteins, [beta]trace - and gammatrace -, were found in CSF, urine and ascitic fluid, while the gammatrace-protein was also demonstrated in serum and pleural fluid. Exhaustive absorption of the antiserum to CSF with human serum removed the antibody to the gammatrace-protein.