HYDROLYSIS OF URINARY NEUTRAL 17-KETOSTEROID CONJUGATES I. COMPARISON OF VARIOUS PROCEDURES
- 1 July 1952
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 10 (3), 221-232
- https://doi.org/10.1530/acta.0.0100221
Abstract
Five procedures for hydrolyzing urines separately and then extracting 17-keto-steroids (17-KS), and 2 for simultaneous hydrolysis and extraction were compared to determine their relative yields of neutral 17-KS and dehydroisoandrosterone-reacting (D-reacting) materials. Max. yields were obtained by 2 methods: (1) by acidifing with 0.1 vol. 70% (v/v) H2SO4; heating in boiling water for 30 min., cooling and extracting with ether; (2) acidifying with 0.1 vol. 40% (v/v) H2SO4, adding benzene, and refluxing for 30 min. Even these methods destroy at least part of the D-reacting substances.Keywords
This publication has 3 references indexed in Scilit:
- QUANTITATIVE DETERMINATION OF DEHYDROISOANDROSTERONEActa Endocrinologica, 1950
- QUANTITATIVE DETERMINATION OF DEHYDROISOANDROSTERONEActa Endocrinologica, 1950
- Colorimetric determination of substances containing the grouping —CH2.CO— in urine extracts as an indication of androgen contentBiochemical Journal, 1938