Metabolic pathways in Ehrlich ascites tumor cells recovering from a low bioenergetic status
- 22 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 350 (2-3), 183-186
- https://doi.org/10.1016/0014-5793(94)00759-4
Abstract
Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightly inhibited by oligomycin. Cells resuspended in a glucose-free medium initially showed high dye uptake and ATP level, which were stable over time: in this case oligomycin caused a drop in both dye uptake and ATP level. The above findings, which are indicative of a marked Crabtree effect in Ehrlich ascites tumor cells, means that it is unlikely that limiting ADP and Pi play an important role in the glucose-induced inhibition of oxidative phosphorylation in this system.Keywords
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