Functional characterization of carrier‐mediated transport of uridine diphosphate N‐acetylglucosamine across the endoplasmic reticulum membrane
- 1 August 1994
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 223 (3), 981-988
- https://doi.org/10.1111/j.1432-1033.1994.tb19076.x
Abstract
Uptake and metabolism of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by rough-endoplasmic-reticulum (rER)-derived vesicles was studied. Analysis of the molecular species, double-label experiments, cis-inhibition and trans-stimulation experiments revealed that uptake represented entry of intact UDP-GlcNAc into the microsomal lumen. The amount of vesicle-associated label at equilibrium uptake was directly proportional to the volume of the intravesicular space, and permeabilized microsomes were unable to retain UDP-GlcNAc. These findings indicate that uptake constituted effective translocation from the medium into the lumen of the vesicles. The microsomal uptake of UDP-GlcNAc met the criteria of bidirectional, carrier-mediated translocation. Transport was time and temperature dependent, saturable, selective, capable of trans-stimulation and operational against a concentration gradient. Uptake studies performed in membrane preparations that were highly enriched in either rER, smooth ER, or Golgi revealed that UDP-GlcNAc was taken up by the ER and by the Golgi apparatus.Keywords
This publication has 23 references indexed in Scilit:
- Bile acid N‐acetylglucosaminidesFEBS Letters, 1990
- Carrier-mediated translocation of uridine diphosphate glucose into the lumen of endoplasmic reticulum-derived vesicles from rat liver.JCI Insight, 1988
- Subcellular organization of glycosylation in mammalian cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1987
- Mechanism of UDP‐sugar transport into intracellular vesiclesFEBS Letters, 1986
- Assay of mannose-6-phosphatase in untreated and detergent-disrupted rat-liver microsomes for assessment of integrity of microsomal preparationsEuropean Journal of Biochemistry, 1986
- Accumulation of UDP‐GlcNAc into intracellular vesicles and occurrence of a carrier‐mediated transportEuropean Journal of Biochemistry, 1985
- Assembly of Asparagine-Linked OligosaccharidesAnnual Review of Biochemistry, 1985
- Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?The Journal of cell biology, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976