Lysostaphin endopeptidase-catalysed transpeptidation reactions of the imino-transfer type

Abstract

The glycylglycine endopeptidase in lysostaphin [of Staphylococcus staphylolyticus] can catalyze hydrolysis and transpeptidation reactions when acting on glycyl peptides. The ability of the enzyme to utilize dansyldiglycine (5-dimethylaminonaphthalene-1-sulfonylglycyl-glycine) as an acceptor molecule in transpeptidation reactions, although it is incapable of hydrolysing the peptide bond in this compound, indicates the enzyme must be capable of forming the equivalent of an imino-enzyme intermediate during the catalytic process.