THE DIABETOGENIC EFFECT OF ALLOXAN AFTER ELIMINATION OF EXTRA-PANCREATIC FACTORS

Abstract

Extra-pancreatic influences were eliminated in that part of the pancreas which was protected by an arterial clamp during the critical first 5 minutes after administration of alloxan. Small doses of buffered alloxan were then injected just inside the clamp portion. Control solutions of the same pH were used to check the significance of ischemia and pH. It was found possible to produce necrosis of the B-cells in the absence of extra-pancreatic influences by only a moderate concentration of alloxan such as could be attained by a diabetogenic injection given intravenously. The exocrine part was not affected. The distribution of islets with necrotic B-cells supports the view that alloxan has a direct effect.