A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes
- 1 February 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 169 (2), 437-440
- https://doi.org/10.1042/bj1690437
Abstract
A simple model of zonal gel filtration is analyzed numerically to establish semiquantitatively how the capacity of a protein-ligand complex to survive gel filtration intact depends on several experimental variables and on the kinetic characteristics of the complex. The design of gel-filtration experiments aimed at detecting and partially purifying binding proteins is discussed.This publication has 11 references indexed in Scilit:
- Binding of bile acids by 100 000g supernatants from rat liverBiochemical Journal, 1977
- Active enzyme gel chromatography: II. Computer simulationsBiophysical Chemistry, 1976
- LigandinBiochemical Society Transactions, 1975
- Fatty Acid-Binding Protein in Small Intestine IDENTIFICATION, ISOLATION, AND EVIDENCE FOR ITS ROLE IN CELLULAR FATTY ACID TRANSPORTJournal of Clinical Investigation, 1974
- Glucocorticoid receptors: Relations between steroid binding and biological effectsJournal of Molecular Biology, 1972
- Mechanism of Action of the Female Sex HormonesAnnual Review of Biochemistry, 1972
- An experimental and theoretical investigation of boundary spreading in gel chromatographyJournal of Chromatography A, 1970
- The application of gel filtration to the study of protein-binding of small moleculesChromatographic Reviews, 1970
- Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anionsJournal of Clinical Investigation, 1969
- PURIFICATION OF AN ESTROPHILIC PROTEIN FROM CALF UTERUSProceedings of the National Academy of Sciences, 1969