Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.
Open Access
- 1 June 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (6), 2679-2683
- https://doi.org/10.1073/pnas.76.6.2679
Abstract
The soluble phase of the cytoplasm of poliovirus-infected [human cervical carcinoma HeLa] cells contains an enzymatic activity able to copy RNA without an added primer. This replicase activity was purified 60-fold; it is absent from uninfected cells. Poly(U) polymerase activity copurifies with replicase activity. Although less pure replicase fractions copy a variety of RNA, purer fractions respond better to poliovirus RNA than to other viral RNA. Even the less pure fractions make a specific copy of the added template, as shown by hybridization of the product to its template RNA but not to other RNA. Among homopolymers only poly(A) .cntdot. oligo(U) was copied by the replicase; other primed homopolymer templates were inactive.This publication has 21 references indexed in Scilit:
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