Differentiation of Dictyostelium discoideum Cells in Suspension Culture
- 1 December 1981
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 127 (2), 301-308
- https://doi.org/10.1099/00221287-127-2-301
Abstract
Differentiation of D. discoideum cells in suspension culture is reported, using a medium containing glucose, albumin, cAMP, EDTA and streptomycin in a phosphate buffer. Production of UDP-galactose:polysaccharide transferase, an enzyme specifically present in prespore cells, and the formation of prespore-specific antigens in more than 60% of the cells occurred. Differentiation in this medium differs from that previously reported with other suspension systems in that cells form only small amorphous agglomerates, there is an absolute requirement for cAMP and prior formation of loose cell mounds on a solid substratum is essential for subsequent differentiation in this medium. This last requirement indicates that the differentiation process, giving rise to the prespore-specific enzyme and antigen, can be resolved into 2 distinct stages, one requiring cell contact on a solid substratum and the other proceeding in small agglomerates incubated in the medium. This medium may be useful for elucidating the role of cAMP and cell contact in slime mold development.This publication has 16 references indexed in Scilit:
- Induction of Cyclic AMP Phosphodiesterase by Disaggregation of the Multicellular Complexes of Dictyostelium discoideumEuropean Journal of Biochemistry, 1979
- Spore Differentiation by Isolated Dictyostelium discoideum Cells, Triggered by Prior Cell ContactDifferentiation, 1978
- Removal by Bovine Serum Albumin of Fatty Acids from Membrane Vesicles and Its Effect on Proline Transport Activity in Escherichia coli1The Journal of Biochemistry, 1978
- The role of cyclic nucleotides and cell agglomeration in postaggregative enzyme synthesis in Dictyostelium discoideumDevelopmental Biology, 1978
- The effects of cyclic AMP on disaggregation-induced changes in activities of developmentally regulated enzymes in Dictyostelium discoideumBiochemical and Biophysical Research Communications, 1978
- Synthesis of developmentally regulated proteins in Dictyostelium discoideum which are dependent on continued cell-cell interactionDevelopmental Biology, 1977
- Separation and biochemical characterization of the two cell types present in the pseudoplasmodium of Dictyostelium mucoroidesJournal of Cell Science, 1977
- Cell differentiation in Dictyostelium under submerged conditions.Proceedings of the National Academy of Sciences, 1977
- Changes in activities of two developmentally regulated enzymes induced by disaggregation of the pseudoplasmodia of DictyosteliumdiscoideumBiochemical and Biophysical Research Communications, 1976
- UDP-GALACTOSE POLYSACCHARIDE TRANSFERASE IN THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM: APPEARANCE AND DISAPPEARANCE OF ACTIVITY DURING CELL DIFFERENTIATIONProceedings of the National Academy of Sciences, 1964