Synthesis and Molecular Recognition of Phosphatidylinositol-3-methylenephosphate

Abstract
Phosphatidylinositol-3-phosphate (PtdIns(3)P) is a spatial regulator of vesicular trafficking and other vital cellular processes. We describe the asymmetric total synthesis of a metabolically stabilized analogue, phosphatidylinositol-3-methylenephosphate (PtdIns(3)MP) from a differentially protected myo-inositol. NMR studies of PtdIns(3)MP bound to the 15N-labeled FYVE domain showed significant 1H and 15N chemical shift changes relative to the unliganded protein.