FAST ALPHA-1-ANTITRYPSIN PHENOTYPING FOR THE DIFFERENTIATION OF PIMM-SUB-TYPES

Abstract

Polyacrylamide gel slabs for isoelectric focusing were adapted for 20 .mu.l [serum] samples by adding urea to the system and letting it run idle before adding the sample. A better separation of protein fractions was obtained, and the distance of the M2- and M8-bands was increased to > 20 mm in a total migration length of 120 mm. A M1M3 standard serum was introduced and the M8 band was included to differentiate PiMM sub-types. The clinical use of the method is demonstrated with the results of a recently published clinical study by Bencze, et al. (1979).