Studies on the Cytophilic Properties of Human β2‐Microglobulin
- 1 February 1979
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 9 (2), 125-134
- https://doi.org/10.1111/j.1365-3083.1979.tb02714.x
Abstract
The mechanism by which exogenously added β2m binds to lymphoid cells has been explored. In the mouse it has been shown that β2m remains associated with plasma membrane macromolecules following solubilization with NP-40 and that approximately 25–30% of the binding could be accounted for by binding to H-2 antigens. No binding to mouse immunoglobulin or Ia antigens could be detected. The sites for binding of the remainder of the cell-hound β2m were not determined. Whereas normal human lymphocytes showed little or no capacity to bind exogenously added β2m, it was found that phytohaemagglutinin (PHA)-stimulated cells could bind β2m. This binding occurred optimally 2 days after PHA stimulation. Approximately half of the binding could be accounted for by binding to HLA antigens. The possible significance of these findings with respect to cellular interactions involving major histocompatibility complex gene products in the immune response is discussed.This publication has 36 references indexed in Scilit:
- The expression and relation of HLA, β2 ‐microglobulin and receptor for marmoset red blood cells on man/mouse and man/Chinese hamster hybrid cellsEuropean Journal of Immunology, 1977
- Role of macrophages in the generation of T helper cells. IV. Nature of genetically related factor derived from macrophages incubated with soluble antigensEuropean Journal of Immunology, 1976
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975
- Inhibition by Anti-beta2-Microglobulin Antisera of Responder Cells and Not of Stimulator Cells in the Mixed Lymphocyte ReactionScandinavian Journal of Immunology, 1975
- Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.The Journal of Experimental Medicine, 1975
- ISOLATION OF HL-A 33,000-DALTON FRAGMENTS CARRYING HIGH HL-A ALLOANTIGENIC ACTIVITYTransplantation, 1975
- Detergent-solubilised H-2 alloantigen is associated with a small molecular weight polypeptideNature, 1974
- RELATIONSHIP BETWEEN HL-A ANTIGENS AND ß2-MICROGLOBULIN AS STUDIED BY IMMUNOFLUORESCENCE ON THE LYMPHOCYTE MEMBRANEThe Journal of Experimental Medicine, 1974
- β ‐ 2 ‐ Microglobulin. Part of the HL‐A Molecule in the Cell MembraneTissue Antigens, 1974
- THYMUS-DERIVED (T) CELL IMMUNOGLOBULINSThe Journal of Experimental Medicine, 1972