STREPTOZOTOCIN-INDUCED PANCREATIC INSULITIS IN MICE - MORPHOLOGIC AND PHYSIOLOGIC STUDIES

Abstract

Pancreatic insulitis and diabetes mellitus were induced in Charles River CD-1 mice with 5 subdiabetogenic injections of streptozotocin. Plasma glucose and immunoreactive insulin levels were measured and animals were sacrificed at intervals for morphologic studies of pancreatic islets and measurements of extractable pancreatic immunoreactive insulin. Light microscopy revealed striking insulitis, 5-6 days after streptozotocin injections, with cell necrosis and eventual islet atrophy due to .beta.-cell depletion. EM demonstrated the presence of infiltrating lymphocytes and macrophages, .beta.-cell necrosis and numerous type C viruses within many of the surviving .beta.-cells. Light microscopic immunoperoxidase stains of islet cell hormones and EM identified relatively increased numbers of .alpha.- and .delta.-cells within the atropic islets 6 and 12 mo. after streptozotocin injections. Plasma glucose, plasma immunoreactive insulin, and extractable pancreatic immunoreactive insulin measurements documented the persistence of profound hyperglycemia, as well as the reduction of plasma and pancreatic immunoreactive insulin levels. Immunofluorescence studies demonstrated the absence of circulating islet cell antibodies during both the acute and chronic stages of the syndrome. The pathogenesis of this model of insulin-deficient diabetes is believed to be a cell-mediated autoimmune reaction directed against pancreatic .beta.-cells altered by subdiabetogenic injections of streptozotocin. The importance of the increased number of type C viruses within surviving .beta.-cells remains obscure.