THE NATURE OF TWO COMPONENTS OF PIG MUCOSAL HEPARIN, SEPARATED BY ELECTROPHORESIS IN AGAROSE GEL
- 30 June 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 45 (7), 1015-1020
- https://doi.org/10.1139/o67-117
Abstract
Two components of pig mucosal heparin were separated by agarose-gel electrophoresis and recovered from the gels. The slower moving component had the higher anticoagulant activity and intrinsic viscosity. The faster moving component had the higher sulfate : carboxyl ratio. Each component was degraded by self-hydrolysis to N-desulfated heparin, deaminated with nitrous acid, and hydrolyzed with formic acid. Two-dimensional electrophoresis and chromatography on thin layers of cellulose showed that each component had two uronic acids with the characteristics of glucuronic and iduronic acids.This publication has 11 references indexed in Scilit:
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