NEW DEOXYURIDINE-5'-TRIPHOSPHATASE IN YOSHIDA SARCOMA-CELLS INVOLVED IN DEOXYURIDINE 5'-TRIPHOSPHATE METABOLISM
- 1 January 1977
- journal article
- research article
- Vol. 37 (6), 1598-1601
Abstract
A Mg independent dUTPase was found in Yoshida sarcoma cells but not in normal rat liver. The phosphatase is specific for dUDP and dUTP, and its Km for dUTP is 2.7 .times. 10-7 M. The enzyme was not inhibited by F and required no divalent cations. It differs from known nucleotide phosphatases. dUMP, which is detectable in a crude extract of normal rat liver, could not be detected in an extract of Yoshida sarcoma cells. With hydroxylapatite column chromatography of the extract, dUMP kinase activity as high as that in normal rat liver was found in fractions separated from the phosphatase activity. The absence of detectable dUMP kinase activity in the crude extract of Yoshida sarcoma cells is due to the presence of this nucleotide phosphatase.This publication has 8 references indexed in Scilit:
- Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase.Proceedings of the National Academy of Sciences, 1966
- New dUTPase and dUDPase activites after infection of Escherichia coli by T2 bacteriophage.Proceedings of the National Academy of Sciences, 1966
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES .2. FORMATION AND INTERCONVERSION OF DEOXYURIDINE PHOSPHATES1963
- DEOXYURIDYLATE KINASE ACTIVITY AND DEOXYURIDINETRIPHOSPHATASE IN ESCHERICHIA COLIProceedings of the National Academy of Sciences, 1962
- Deoxycytidine Di- and Triphosphate Cleavage by an Enzyme Formed in Bacteriophage-infected Escherichia coliJournal of Biological Chemistry, 1961
- Studies on the biosynthesis of deoxyribonucleic acid by extracts of mammalian cells IV. The phosphorylation of thymidineBiochimica et Biophysica Acta, 1960
- Protein chromatography on calcium phosphate columnsArchives of Biochemistry and Biophysics, 1956
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951