Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase.
- 1 October 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (4), 1233-1240
- https://doi.org/10.1073/pnas.56.4.1233
Abstract
Data have been presented indicating that gene 56 of bacteriophage T4 controls the synthesis of both the phage-induced dCTPase and dUTPase activities. Fractionation of extracts of phage-infected cells with streptomycin sulfate, DEAE-cellulose chromatography, and hydroxylapatite chromatography indicated that these two activities probably reside in the same protein. Mutual inhibition studies indicated that the same site may catalyze both reactions. Furthermore, the purified enzyme also hydrolyzes dUDP, and this dUDPase activity is not induced in E. coli by gene 56 mutants. The dUDP inhibits dCTP hydrolysis, suggesting that there is a common catalytic site for dUTP, dCTP, and dUDP hydrolysis on the phage-induced enzyme. The results of others would imply that dCDP is also hydrolyzed at this site.This publication has 14 references indexed in Scilit:
- The synthesis of deoxycytidylate deaminase and dihydrofolate reductase and its control in Escherichia coli infected with bacteriophage T4 and T4 amber mutantsVirology, 1966
- Mutants of bacteriophage T4 unable to cause breakdown of host DNA.Proceedings of the National Academy of Sciences, 1966
- Morphogenesis of bacteriophage T4 in extracts of mutant-infected cells.Proceedings of the National Academy of Sciences, 1966
- The enzymology of virus-infected bacteria. IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2.1963
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES .2. FORMATION AND INTERCONVERSION OF DEOXYURIDINE PHOSPHATES1963
- EARLY ENZYME SYNTHESIS AND ITS CONTROL IN E. COLI INFECTED WITH SOME AMBER MUTANTS OF BACTERIOPHAGE T4Proceedings of the National Academy of Sciences, 1962
- DEOXYURIDYLATE KINASE ACTIVITY AND DEOXYURIDINETRIPHOSPHATASE IN ESCHERICHIA COLIProceedings of the National Academy of Sciences, 1962
- Deoxycytidine Di- and Triphosphate Cleavage by an Enzyme Formed in Bacteriophage-infected Escherichia coliJournal of Biological Chemistry, 1961
- Deoxycytidine Triphosphatase, an Enzyme Induced by Bacteriophage InfectionJournal of Biological Chemistry, 1960
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. INFLUENCE OF BACTERIOPHAGE T2 ON THE SYNTHETIC PATHWAY IN HOST CELLSProceedings of the National Academy of Sciences, 1959