Sequencing and the alignment of structural genes in the nqr operon encoding the Na+‐translocating NADH‐quinone reductase from Vibrio alginolyticus
- 17 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 363 (1-2), 75-77
- https://doi.org/10.1016/0014-5793(95)00283-f
Abstract
We previously cloned a part of nqr operon encoding the Na+-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus (Hayashi et al., FEBS Lett. 356 (1994) 330–332]. From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the γ-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the β-subunit (45.3 kDa) were recognized. The gene encoding the α-subunit was located upstream, and together with the recent report by Beattie et al. [FEBS Lett. 356 (1994) 333–338], the nqr operon was found to be constructed from six consecutive structural genes, where nqrl, nqr3 and nqr6 correspond to the α-, γ-, and β-subunits, respectively, of the NQR complexKeywords
This publication has 13 references indexed in Scilit:
- Cloning and sequencing of four structural genes for the Na+‐translocating NADH‐ubiquinone oxidoreductase of Vibrio alginolyticusFEBS Letters, 1994
- Cloning of the Na+‐translocating NADH‐quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the β‐subunit in Escherichia coliFEBS Letters, 1994
- Correlation between the respiration-driven Na+ pump and Na+-dependent amino acid transport in moderately halophilic bacteriaJournal of General Microbiology, 1993
- The Gene Locus of the Proton-translocating NADH : Ubiquinone Oxidoreductase in Escherichia coliJournal of Molecular Biology, 1993
- Primary structures of two subunits of NADH:ubiquinone reductase from Neurospora crassa concerned with NADH-oxidation. Relationship to a soluble NAD-reducing hydrogenase of Alcaligenes eutrophusBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1991
- The NADH-binding subunit of the energy-transducing NADH-ubiquinone oxidoreductase of Paracoccus denitrificans: gene cloning and deduced primary structureBiochemistry, 1991
- Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenaseBiochemistry, 1991
- Sodium-transport NADH-quinone reductase of a marineVibrio alginolyticusJournal of Bioenergetics and Biomembranes, 1989
- Subunit component and their roles in the sodium-transport NADH: quinone reductase of a marine bacterium, Vibrio alginolyticusBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Characterization of the Na+-dependent respiratory chain NADH:quinone oxidoreductase of the marine bacterium, Vibrio alginolyticus, in relation to the primary Na+ pumpBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1984