Preparation of Escherichia coli cell extract for highly productive cell-free protein expression
Top Cited Papers
- 1 March 2004
- journal article
- Published by Springer Nature in Journal of Structural and Functional Genomics
- Vol. 5 (1/2), 63-68
- https://doi.org/10.1023/b:jsfg.0000029204.57846.7d
Abstract
As structural genomics and proteomics research has become popular, the importance of cell-free protein synthesis systems has been realized for high-throughput expression. Our group has established a...This publication has 24 references indexed in Scilit:
- Expression of soluble recombinant proteins in a cell-free system using a 96-well formatJournal of Biochemical and Biophysical Methods, 2003
- An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free systemProceedings of the National Academy of Sciences, 2002
- An unnatural base pair for incorporating amino acid analogs into proteinsNature Biotechnology, 2002
- Cell-free translation reconstituted with purified componentsNature Biotechnology, 2001
- Structural genomics projects in Japan.Nature Structural & Molecular Biology, 2000
- Prolonging Cell-Free Protein Synthesis by Selective Reagent AdditionsBiotechnology Progress, 2000
- A Highly Efficient Cell‐Free Protein Synthesis System from Escherichia coliEuropean Journal of Biochemistry, 1996
- A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High YieldScience, 1988
- ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purificationJournal of Bacteriology, 1988
- Cloning and expression of the gene for bacteriophage T7 RNA polymerase.Proceedings of the National Academy of Sciences, 1984