THE TURNOVER OF CYCLIC-AMP IN CULTURED FIBROBLASTS
- 1 January 1980
- journal article
- research article
- Vol. 6 (1), 3-14
Abstract
The determination of the turnover of c[cyclic]AMP in [human] WI-38 and VA13 [neoplastic WI-38] cultured fibroblasts stimulated by prostaglandin E1 is reported. The method made use of data obtained from a process of continuously labeling the cellular adenine nucleotide pools by incubation with [3H]-adenine. The cAMP turnover was estimated from the delay in appearance of tritium label in the cAMP pool as compared to the cellular ATP. For WI-38 cells, the half-life of cAMP when accumulation had reached a steady-state, was 1.46 min; in the presence of 0.5 mM 1-methyl-3-isobutylxanthine the half-life was increased to 9.24 min. For VA13 transformed fibroblasts the half-life of cAMP determined by this method was 6.30 min. cAMP in these latter cells in the absence of hormone had a half-life of 3.01 min. The hormone evidently has profound effects on phosphodiesterase as well as adenylate cyclase activities in these cells.This publication has 4 references indexed in Scilit:
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