Surface Structure of Spermatozoa Frozen for Artificial Insemination*
- 24 April 2009
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 9 (2), 155-161
- https://doi.org/10.1111/j.1439-0272.1977.tb01275.x
Abstract
Samples of human semen frozen in liquid nitrogen ( - 196 degrees C) with no glycerol, 5 and 10% glycerol were compared with samples that were untreated, with 10% glycerol but not frozen, and spermatozoa frozen at -20 degrees C. SEM and TEM of the samples indicates that 10% glycerol caused fewer surface changes of the spermatozoa than other treatments. Motility counts after the various freezing treatments were also highest when 10% glycerol was used as the cryoprotectant. Nonetheless, cryopreservation is detrimental to spermatozoa and often causes considerable damage to the acrosome with a leakage of the acrosomal contents.Keywords
This publication has 12 references indexed in Scilit:
- ACROSOMAL ENZYMES: IMMUNOCHEMICAL LOCALIZATION OF ACROSIN AND HYALURONIDASE IN RAM SPERMATOZOAReproduction, 1975
- THE EFFECTS OF WASHING ON THE ULTRASTRUCTURE AND CYTOCHEMISTRY OF RAM SPERMATOZOAReproduction, 1974
- VASECTOMY: LONG-TERM EFFECTS IN THE RHESUS MONKEYReproduction, 1972
- Comparative studies on the proteolysis of fixed gelatin membranes by mammalian sperm acrosomesJournal of Anatomy, 1972
- Effect of freezing on the molecular weight of bacterial DNACryobiology, 1972
- Variation Due To Freezing In The Citric Acid Content Of Human SemenFertility and Sterility, 1971
- HYALURONIDASE IN HUMAN SEMEN AND SPERM SUSPENSIONS SUBJECTED TO TEMPERATURE SHOCK AND TO FREEZINGReproduction, 1970
- Freeze preservation of human spermAmerican Journal of Obstetrics and Gynecology, 1969
- DNA CONTENT OF HUMAN SPERMATOZOA AFTER STORAGE AT LOW TEMPERATURESReproduction, 1968
- The Spermicidal Powers of Chemical Contraceptives. IV. More Pure SubstancesEpidemiology and Infection, 1932