Analysis of B6 Vitamers by Micellar Electrokinetic Capillary Chromatography with Laser-Excited Fluorescence Detection
- 1 August 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 24 (8), 347-351
- https://doi.org/10.1093/chromsci/24.8.347
Abstract
Micellar electrokinetic capillary chromatography (MECC) involves the application of a high voltage (10 to 40 kV) across a capillary column (25 to 75 μm i.d.) that is filled with a solution containing micelles. The mobile phase in this work consists of sodium dodecyl sulfate in an aqueous phosphate/borate buffer system. Pyridoxine (vitamin B6) and five of its metabolites are separated, with efficiencies as high as 60,000 theoretical plates/meter. Pyridoxic acid, a metabolite of B6, is separated and quantitated in human urine using laser-excited fluorescence detection. Limits of detection are less than a picogram injected.This publication has 7 references indexed in Scilit:
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