The Effects of Hemin and Double-Stranded RNA on α and β Globin Synthesis in Reticulocyte and Krebs II Ascites Cell-Free Systems and the Relationship of These Effects to an Initiation Factor Preparation
- 1 July 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (7), 2863-2866
- https://doi.org/10.1073/pnas.71.7.2863
Abstract
Protein synthesis in reticulocyte lysates ceases abruptly in the absence of added hemin or in the presence of double-stranded RNA. A similar effect of double-stranded RNA is observed in Krebs II ascites cell-free systems translating exogenous globin mRNA. The shut-off of protein synthesis is due to inhibition of initiation and can be prevented or reversed by addition of the initiation factor preparation M(3). Preparations of M(1), M(2), and dissociation factor are ineffective under these conditions. The effects of added hemin, M(3), and globin mRNA on the synthesis of alpha and beta globin chains have been studied in the reticulocyte and ascites cell extracts. When the concentration of M(3) is rate limiting, the synthesis of beta chains exceeds that of alpha chains. When the concentration of mRNA is rate limiting, synthesis of alpha and beta chains is more nearly equal.Keywords
This publication has 29 references indexed in Scilit:
- The control of hemoglobin synthesis. A comparison of the role of heme in rabbit bone marrow and reticulocytes.1972
- Translation of Exogenous Messenger RNA for Hemoglobin on Reticulocyte and Liver RibosomesProceedings of the National Academy of Sciences, 1971
- Cytoplasm from Poliovirus-infected HeLa Cells inhibits Cell-free Haemoglobin SynthesisNature New Biology, 1971
- Studies on cessation of protein synthesis in a reticulocyte lysate cell-free systemBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Translational Control in Hemoglobin SynthesisCold Spring Harbor Symposia on Quantitative Biology, 1969
- The Role of Heme in the Synthesis and Assembly of HemoglobinJournal of Biological Chemistry, 1968
- Factors affecting the rate of protein synthesis in lysate systems from reticulocytesArchives of Biochemistry and Biophysics, 1968
- Hemoglobin synthesis during amphibian metamorphosis: I. Chemical studies on the hemoglobins from the larval and adult stages of Rana catesbeianaJournal of Molecular Biology, 1968
- Stimulation of globin-chain initiation by hemin in the reticulocyte cell-free system.Proceedings of the National Academy of Sciences, 1968
- The stimulation of globin synthesis by heme.Proceedings of the National Academy of Sciences, 1966