Hras1-selected, chromosome-mediated transformants vary in phenotypein vitro and tumorigenic potentialin vivo
- 15 October 1986
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 38 (4), 603-612
- https://doi.org/10.1002/ijc.2910380422
Abstract
Transfection of mouse C127 cells with mitotic chromosomes isolated from a human EJ bladder carcinoma cell line gave rise, at high frequency, to foci of transformed cells. Independent, HRASI-selected chromosome-mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony-forming abilities in low-melting-point agarose. Subcutaneous inoculation of neonatally thymectomized, Ara-C-protected, total-body-irradiated CBA mice was used to compare the tumorigenic potential of each transformant. Significant quantitative and qualitative differences in tumorigenicity were found between transformants which correlated with differences in malignant phenotype observed in vitro. The sensitivity of the tumorigenicity assay is such that rare transformation events can be selected directly in vivo.This publication has 44 references indexed in Scilit:
- The gross pathology and histological features of tumours produced by inoculation of human cell lines into immune‐deprived miceThe Journal of Pathology, 1986
- Structure of bovine papillomavirus type 1 DNA in a transformed mouse cell lineJournal of Molecular Biology, 1986
- Trends in oncogenesTrends in Genetics, 1985
- A transforming activity not detected by DNA transfer to NIH 3T3 cells is detected by JB6 mouse epidermal cells.Molecular and Cellular Biology, 1985
- New human transforming genes detected by a tumorigenicity assay.Molecular and Cellular Biology, 1984
- New Method for Detecting Cellular Transforming GenesScience, 1982
- Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.Proceedings of the National Academy of Sciences, 1982
- Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cellsNature, 1982
- A quantitative in vitro focus assay for bovine papilloma virusVirology, 1980
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973