The Utility of Cyclodextrin in Mobile Phase for High-Performance Liquid Chromatographic Separation of Isomeric Estrogens
- 1 May 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 11 (7), 1475-1484
- https://doi.org/10.1080/01483918808067187
Abstract
Cyclodextrin was used as a component of mobile phase for the separation of isomeric estrogens by reversed-phase high-performance liquid chromatography. The positional isomers of catechol and guaiacol estrogens were distinctly resolved by the addition of β-cyclodextrin in the mobile phase. The separation of estriol 16- and 17-glucuronides requires usually a prolonged time. The use of β-cyclodextrin in the mobile phase, however, reduced the retention time considerably. The effect of β-cyclodextrin concentration in the mobile phase on the detector response was also investigated. The response of a fluorescence detector was raised with an increasing concentration of β-cyclodextrin, while that of an electrochemical detector was significantly depressed.This publication has 12 references indexed in Scilit:
- Improved Cyclodextrin Chiral Phases: A Comparison and ReviewJournal of Liquid Chromatography, 1986
- Liquid chromatographic separation of diastereomers and structural isomers on cyclodextrin-bonded phasesAnalytical Chemistry, 1985
- Cyclodextrin Bonded Phases For the Liquid Chromatographic Separation of Optical, Geometrical, and Structural IsomersJournal of Chromatographic Science, 1984
- Increase in prostaglandin's levels in plasma and thecal layer of preovulatory follicles in relation to mid-sequence qvipositionJournal of Steroid Biochemistry, 1984
- Fluorescence Behavior of 7-Substituted Coumarin Derivatives by Inclusion Complexation with β-CyclodextrinYAKUGAKU ZASSHI, 1983
- Studies on Steroids. CLXXVIII. Separation of Estrogen Glucuronides by High-Performance Liquid ChromatographyJournal of Liquid Chromatography, 1981
- The simultaneous determination of the products of estrogen 2- and 4-hydroxylase action; The use of high-performance liquid chromatography with electrochemical detectionAnalytical Biochemistry, 1981
- Liquid chromatographic elution characteristics of some solutes used to measure column void volume on C18 bonded phasesAnalytical Chemistry, 1981
- Applications of Cyclodextrins in Chromatographic Separations and Purification MethodsSeparation and Purification Methods, 1981
- Enhancement of fluorescence intensity of dansyl amino acids on silica gel plate using cycloheptaamylose.CHEMICAL & PHARMACEUTICAL BULLETIN, 1975