Proton magnetic resonance melting studies of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp
- 18 January 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (2), 269-277
- https://doi.org/10.1021/bi00271a006
Abstract
PMR melting curves are reported for the base and ribose 1'' nonexchangeable protons of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp. In general, the double helices melt in a cooperative 2-state transition. For CCGGp, CCGGAp, CCGGUp, and ACCGGUp, the 3'' ribose exhibits conformational flexibility at lower temperatures than the other ribose residues. For ACCGGp, the 5''-C ribose is the first to exhibit flexibility. The conformations of the 3'' terminal bases in CCGGAp and CCGGUp change cooperatively with the double- to single-strand transition, whereas the 5'' A of ACCGGp appears to have more conformational freedom.This publication has 9 references indexed in Scilit:
- Nuclear Overhauser studies of CCGGAp, ACCGGp, and ACCGGUpBiochemistry, 1983
- DNA and RNA oligomer thermodynamics: The effect of mismatched bases on double‐helix stabilityBiopolymers, 1981
- Sequence and structure in double-stranded ribonucleic acid: (A-G-C-U)2 and (A-C-G-U)2Biochemistry, 1981
- Comparative study of ribonucleotides, deoxyribonucleotides, and hybrid oligonucleotide helixes by nuclear magnetic resonanceBiochemistry, 1981
- Unusual structures in single-stranded ribonucleic acid: Proton nuclear magnetic resonance of AUCCA in deuterium oxideBiochemistry, 1981
- Conformational flexibility of the 3' acceptor end of transfer ribonucleic acidBiochemistry, 1980
- Conformation studies of 13 trinucleoside diphosphates by 360 MHz PMR spectroscopy. a bulged base conformationBiophysical Chemistry, 1980
- Helix-Coil Transition of the Self-Complementary dG-dG-dA-dA-dT-dT-dC-dC DuplexEuropean Journal of Biochemistry, 1979
- Nucleotide rigidityNature, 1976