Structural analysis of the specific polysaccharide of Streptococcus pneumoniae type 9L (American type 49)

Abstract
The specific capsular polysaccharide produced by Streptococcus pneumoniae type 9L (American type 49) is composed of D-galactose (1 part), D-glucose (1 part), D-glucuronic acid (1 part), 2-acetamido-2-deoxy-D-mannose (1 part) and 2-acetamido-2-deoxy-D-glucose (1 part). Partial acid hydrolysis, periodic oxidation, nitrous acid deamination, optical rotation, methylation and 13C and 1H NMR studies showed that the polysaccharide is an unbranched high MW linear polymer of a repeating pentasaccharide unit.