South african pilchard oil. 5. The isolation and structure of an eicosapentaenoic acid from South African pilchard oil
- 1 July 1956
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 63 (3), 469-475
- https://doi.org/10.1042/bj0630469
Abstract
Cis-Eicosapenta-5:8:ll:14:17-enoic acid was isolated from South African pilchard oil by the techniques of lithium salt-acetone segregation, urea-complex fractionation, molecular distillation and reversed-phase partition chromatography. Some properties of this acid were determined. The position of the double bond nearest to the carboxyl group was definitely determined by using an Arndt-Eistert synthesis in combination with a method of oxonolytic degradation. This procedure is probably of wide applicability. The degree of purity of the acid was critically considered.Keywords
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