A PRACTICAL METHOD FOR ESTIMATION OF URINARY PREGNANEDIOL AND ALLOPREGNANEDIOL USING THIN LAYER CHROMATOGRAPHY

Publisher Website
Google Scholar
Abstract
A method is described for the assay of urinary pregnanediol and allo-pregnanediol which employs acid hydrolysis of pregnanediol glucuronide, ascending thin-layer chromatography and spectrophotometric quantiation of the eluted pregnanediol and allopregnanediol. The reliability of the method was established by analysis of infra-red and UV absorption spectra, melting point determination, recovery experiments, duplicate assays and comparison with the Klopper method. The method has advantages in that it is rapid and requires small urine samples.