The Relationship between Colony-forming Ability and Chromosomal Aberrations Induced in Human T-lymphocytes after γ-Irradiation
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 58 (2), 293-301
- https://doi.org/10.1080/09553009014551631
Abstract
Using aliquots from the same samples of irradiated normal human blood, T-lymphocyte survival was measured by (a) 14-day colony growth, (b) the proportion of chromosomally normal first post-irradiation metaphases and (c) the proportion of cells not suffering interphase death and delay within 48 h after irradiation. Combining (b) and (c) gives a prediction of the proportion of cytogenetically undamaged cells which reach metaphase after 48 h. These cells should be capable of producing viable colonies under suitable culture conditions. A comparison of these values with those observed in (a) shows a reasonable agreement in accordance with the hypothesis that gross chromosomal damage and cell reproductive death are closely related.This publication has 24 references indexed in Scilit:
- Radiation sensitivity of T-lymphocytes grown with recombinant human interleukin-2Mutation Research Letters, 1987
- In vitro radiosensitivity of human fresh T-lymphocytes by colony formation assay using PHA and recombinant interleukin-2.Journal of Radiation Research, 1987
- Rapid estimation of the duration of .GAMMA.-ray-induced proliferation delay in human lymphocytes by the harlequin staining technique.RADIOISOTOPES, 1984
- Radiation senstitivity of human lyphocytesMutation Research, 1982
- The use of harlequin staining to measure delay in the human lymphocyte cell cycle induced by in vitro X-irradiationMutation Research, 1980
- Ionizing Radiation and the Immune ResponseAdvances in Immunology, 1976
- Chromosome aberration dosimetry using human lymphocytes in simulated partial body irradiationPhysics in Medicine & Biology, 1973
- The fate of chromosome aberrationsJournal of Theoretical Biology, 1973
- Chromosomal Aberrations and Mortality of X-Irradiated Mammalian Cells: Emphasis on RepairProceedings of the National Academy of Sciences, 1971
- The Role of Genetic Damage in Radiation-Induced Cell LethalityPublished by Elsevier ,1966