Human Platelet Actin
- 1 December 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 81 (3), 571-577
- https://doi.org/10.1111/j.1432-1033.1977.tb11984.x
Abstract
Human blood platelet action was purified using 30% sucrose to extract actomyosin and KI to dissociate actomyosin and to depolymerize actin. Pure actin thus obtained resembles skeletic muscle actin in its polymerization properties, CD [circular dichroism] spectra and ability to activate myosin Mg2+-ATPase. Isoelectric focusing gel analysis shows that human blood platelet actin exists in .beta. and .gamma. forms. The ratio of .beta. to .gamma. forms is 5:1 in purified actin, whole cell extract and in all fractions studied.This publication has 24 references indexed in Scilit:
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