Enzyme immunoassay for methamphetamine.
- 1 January 1983
- journal article
- research article
- Published by Pharmaceutical Society of Japan in Journal of Pharmacobio-Dynamics
- Vol. 6 (1), 33-38
- https://doi.org/10.1248/bpb1978.6.33
Abstract
A competitive enzyme immunoassay for methamphetamine [a drug of abuse] with alkaline phosphatase labeled methamphetamine. Sepharose-antibody and p-nitrophenylphosphate as substrate was developed. The anti-methamphetamine antisera produced in rabbits by immunization with N-(4-aminobutyl) methamphetamine-BSA [bovine serum albumin] conjugate were specific for methamphetamine and showed low cross-reactivities with p-OH methamphetamine and amphetamine (metabolites of methamphetamine). The range of methamphetamine measurable by the enzyme immunoassay was 1-300 ng/tube. According to the assay, methamphetamine could be detected from [human] urine and extract of hair.This publication has 3 references indexed in Scilit:
- A rapid and simple screening method for methamphetamine in urine by radioimmunoassay using a 125I-labeled methamphetamine derivative.CHEMICAL & PHARMACEUTICAL BULLETIN, 1980
- Enzyme immunoassay for phenobarbital.CHEMICAL & PHARMACEUTICAL BULLETIN, 1980
- The radioimmunoassay for methamphetamine.CHEMICAL & PHARMACEUTICAL BULLETIN, 1977