Glycogenolytic Effect of Adenosine 3',5'-Monophosphate in the Canine Liver

Abstract

A nucleotide, cyclic adenosine 3[image],5[image]-monophosphate (3[image],5[image]-AMP), had glycogenolytic properties when perfused through the portal vein of the intact tricannulated dog. Following intraportal perfusion of 2-6 mg/kg of cyclic 3[image],5[image]-AMP there was a mean increase in hepatic vein blood glucose concentration of 20 mg/100 ml between 5 and 10 min. There were blood glucose concentration rises of a lesser magnitude in the splenic artery and portal vein. Suspension of cyclic 3[image],5[image]-AMP in 0.1 M acetate buffer with 6 mM of Mg at pH 5.08 increased the glycogenolytic responsiveness of this system, with a mean increase in hepatic vein glucose concentration of 52 mg/100 ml. Positive hepatic -portal vein differences were noted in all perfusions of cyclic 3[image],5[image]-AMP. Adenosine 5[image]-monophosphate, adenosine 5[image]-diphosphate, adenosine 5[image]-triphosphate and buffer alone had no apparent glycogenolytic effect when perfused intraportally in the dog.