Synthesis of deoxyoligonucteotides containing 7-deazaadenine: recognition and cleavage by restriction endonucleaseBglII andSau3AI (Nucleosides and Nucleotides Part 551)
- 11 December 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (23), 8939-8949
- https://doi.org/10.1093/nar/12.23.8939
Abstract
Deoxydecanucleotides having a recognition sequence of Bgl II and Sau 3AI, and their 7-deazaadenine analogs were synthesized. The decanucleotides containing 7-deazaadenine in place of adenine were partially resistant to the hydrolysis by Sau 3AI and strongly resistant to that by Bgl II. A new hypothesis on the mode of recognition and cleavage of specific nucleotide sequences by Bgl II, recognizing one strand and cleaving the other strand, is presented.Keywords
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