Transduction of Streptomycin Sensitivity into Resistant Mutants of Salmonella typhimurium

Abstract

The infection of slow-growing one-step intermediate streptomycin resistant mutants with phage from wild-type bacteria, streptomycin indifferent (one-step completely resistant) bacteria, or other intermediate resistant mutants gives rise to fast-growing streptomycin sensitive transductants. Apparently slow growth and streptomycin resistance are controlled by a single locus. It was possible to cross one-step resistants with resistant, indifferent and sensitive chains. The results indicate that intermediate resistance is controlled by multiple loci not linked to the indifference locus.