A NEW METHOD FOR THE COLORIMETRIC DETERMINATION OF ALDOSTERONE IN URINE
- 1 June 1957
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 25 (2), 161-172
- https://doi.org/10.1530/acta.0.0250161
Abstract
Aldosterone from adrenal cortical extracts has an important function in the regulation of electrolyte and water metabolism. Previous methods of determination were very time consuming and required large groups of animals; therefore the authors developed a new chemical determination procedure. Urinary extracts were prepared by extraction with chloroform and purification in two paperchromatography systems. A colored compound, estimated by spectrophotometrical procedures, was formed by reacting with 2,4-dinitrophenylhydrazine. Normal values for the aldosterone excretion were 5.5-13.0 [mu]g/24 hours in men and 2.5-8.0 [mu]g/24 hours in women.Keywords
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