Effects of Se‐depletion on glutathione peroxidase and selenoprotein W gene expression in the colon
- 27 December 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (3), 792-796
- https://doi.org/10.1016/j.febslet.2004.12.042
Abstract
Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60–83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17–25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletionKeywords
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