An Evaluation of Fluorometric Substrates for Lipase
- 1 January 1968
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 1 (9), 551-563
- https://doi.org/10.1080/00032716808051162
Abstract
The results of a complete study of 12 substrates for lipase indicated 4-methyl umbelliferone heptanoate to be the best substrate for the analysis of this enzyme. Using this ester, from 0.000020 to 0.066 units per ml. of porcine pancreas lipase can be determined with an accuracy and precision of about 1.5%. Analysis is performed by a direct initial reaction rate method in 2–3 minutes.Keywords
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