Post‐transcriptional Modifications of Oat Coleoptile Ribonucleic Acids
- 1 February 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 104 (1), 271-277
- https://doi.org/10.1111/j.1432-1033.1980.tb04425.x
Abstract
Evidence is presented for the occurrence of 5''-terminal capping structure in poly(A)-rich RNA from oat coleoptile tissue. Similar to the cap structures in mRNA from other eukaryotic organisms, the 5'' terminus of these oat coleoptile RNA molecules consists of 7-methylguanosine joined 5'' to 5'' with the adjacent (penultimate) nucleoside by means of 3 phosphate groups in 2 pyrophosphate linkages. The penultimate nucleoside contains primarily purine bases, but small amounts of pyrimidines (cytidine) are also detectable. Some monophosphorylated 5''-termini were also detected, but they appear to occur as a result of RNA degradation. In addition to the 5'' cap, oat RNA molecules are also post-transcriptionally modified with a low frequency of N6-methylations of internal adenosines.Keywords
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