PREGNANETRIOL IN URINE: DETERMINATION AS A 17-KETOGENIC STEROID
- 1 December 1959
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. XXXII (IV), 596-605
- https://doi.org/10.1530/acta.0.xxxii0596
Abstract
Pregnanetriol is determined by the oxidation of its glucuronide to aetiocholanolone which is determined as a Zimmermann chromogenfollowing chromatography on silica gel. The method indicated a difference between normal individuals and patients with congenital adrenal hyperplasia, and the results were compared with a method using enzymic hydrolysis of the steroid conjugate.Keywords
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