A SIMPLE METHOD OF PREPARING AN ANTIGEN FROM HISTOPLASMA CAPSULATUM FOR USE IN THE COMPLEMENT FIXATION TEST
- 1 August 1955
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 1 (7), 520-524
- https://doi.org/10.1139/m55-065
Abstract
A simple method for preparation of complement fixing antigen from Histoplasma capsulatum is outlined. The procedure consists of extraction of the sedimented yeast phase growth with pyridine for two hours and subsequent washing of the extracted sediment three times with buffered saline solution. The antigen so prepared is non-viable, potent, stable, and not anticomplementary; it appears to be suitable for use in routine diagnostic work.Keywords
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