STAINING OF OSMIUM FIXED VESTOPAL EMBEDDED TISSUE SECTIONS FOR LIGHT MICROSCOPY

Abstract

A new general staining technique for light microscopy of osmium fixed Vestopal embedded tissue sections employing cobalt sulfide is described. The staining is carried out by floating 1-1.5[mu] thick sections mounted on Formvar coated grids upon drops of the staining solutions. The method involves attachment of calcium compounds to certain tissue elements and the conversion of these calcium compounds to cobalt sulfide as in the Gomori technique for alkaline phosphatase. Furthermore the PAS-reaction was adapted to the "grid-drop" technique, and satisfactory results were achieved.