Physical linkage of mouse lambda genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences.
Open Access
- 1 February 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (2), 711-718
- https://doi.org/10.1128/mcb.9.2.711
Abstract
The first complete map of a mammalian immunoglobulin gene locus is presented. Mouse lambda genes were mapped by pulsed-field gel electrophoresis. The gene order is V2-Vx-C2-C4-V1-C3-C1. The distance between V2 or Vx and the C2-C4 cluster is 74 or 55 kilobases (kb), respectively, whereas that between V1 and C3-C1 is only 19 kb; V2 and C3-C1 are at least 190 kb apart. Thus, the distances between the lambda subloci are inversely proportional to their frequencies of rearrangement. The related gene lambda 5 is not within the 500 kb of the lambda locus mapped here.This publication has 38 references indexed in Scilit:
- A new variable region in mouse immunoglobulin lambda light chains.The Journal of Experimental Medicine, 1987
- A new V gene expressed in lambda‐2 light chains of the mouseEuropean Journal of Immunology, 1987
- Role of DNA topology in Mu transposition: Mechanism of sensing the relative orientation of two DNA segmentsCell, 1986
- EcoK restriction during in vitro packaging of coliphage lambda DNAGene, 1985
- Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell linesNature, 1984
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Immunoglobulin GenesAnnual Review of Immunology, 1983
- Structural alterations in J regions of mouse immunoglobulin λ genes are associated with differential gene expressionNature, 1982
- Sequences of mouse immunoglobulin light chain genes before and after somatic changesCell, 1978
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975